Where is pgal formed

Or, a fragment containing the spot can be cut from the sheet and chemically analyzed. To determine which, if any, of the substances separated on the chromatogram are radioactive, a sheet of X-ray film is placed next to the chromatogram. If dark spots appear on the film because of radiation emitted by the 14 C atoms , their position can be correlated with the positions of the chemicals in the chromatogram.

Using this technique of autoradiography , Calvin found that 14 C turned up in glucose molecules within 30 seconds after the start of photosynthesis. When he permitted photosynthesis to proceed for only 5 seconds, however, the radioactivity was concentrated in several other, smaller, molecules.

Apparatus used to follow the fate of 14 CO 2 in the dark reactions. The algal suspension is placed in the "lollypop", supplied with 14 CO 2 , and illuminated. The resulting 6-carbon compound breaks down into two molecules of 3-phosphoglyceric acid PGA. Phosphoglyceraldehyde serves as the starting material for the synthesis of glucose and fructose. Glucose and fructose make the disaccharide sucrose , which travels in solution to other parts of the plant e.

Glucose is also the monomer used in the synthesis of the polysaccharides starch and cellulose. Calvin's Experiment Figure 4. James A. Bassham The experimental apparatus is shown above.

Figure 4. Click to see full answer. Beside this, what is Pgal in photosynthesis? Furthermore, what are two uses for the Pgal produced during the Calvin cycle? Describe two ways of the energy of ATP is used in the Calvin cycle. RuBP stands for ribulose bisphosphate, it's a 5 carbon compound involved in the Calvin cycle , which is part of the light independent reactions of photosynthesis. The recycled molecule is ribulose bisphosphate RuBP. Examine figure 2 and locate these molecules in the cycle.

Glucose phosphate is one result of PGAL metabolism; it is a common energy molecule. Two almost simultaneous reactions occur next: an oxidation and a phosphorylation. The phosphorylation, using some of the oxidation energy, attaches an inorganic phosphate group P i to the carbon ring the bond energy is similar to that of the high energy bonds of ATP.

Two ATPs were needed to activate the pathway, so at this point the energy use and production are equal. Remember that the above reactions take place in the cytosol of cells and are catalyzed by enzymes specific for each reaction.